HPLC Column, HPS Silica, 5um, 2.1mm ID x 50mm Length, 120A. Cogent Brand. 1 EA.

Additional Info:

75001-05P-2 Cogent HPS Silica™ HPLC Column is built with a high‑purity, unmodified silica stationary phase, providing reliable and reproducible performance for normal phase chromatography and USP L3 methods.

Unlike bonded phases, HPS Silica™ is chemically unmodified, allowing strong interactions with polar and hydrophilic compounds, making it ideal for lipid, fat, and other normal phase separations.

This proprietary silica has been widely used in HPLC laboratories for years, offering consistent performance and serving as a dependable alternative to commonly used silica columns such as Inertsil® and Luna®.

The 5 µm particle size provides a balance of efficiency and durability, allowing operation at higher flow rates with lower backpressure, making it well‑suited for routine analytical methods.

50 mm Length – Fast Normal Phase Separations

The 50 mm column length is optimized for speed and efficiency.

  • Short run times for rapid analysis

  • Reduced solvent consumption

  • Ideal for method development and screening

✅ Recommended for fast normal phase HPLC and high‑throughput workflows

2.1 mm ID – Low Flow Analytical Format

  • Designed for 2.1 mm ID analytical columns

  • Reduces solvent consumption

  • Ideal for LC‑MS compatible workflows

  • Maintains sensitivity in low‑flow systems

✅ Recommended for labs seeking solvent savings and efficient separations

Flow Rate & System Requirements (2.1 mm ID, 5 µm)

⚠️ Requires low‑flow capable HPLC or UHPLC system

  • Typical flow rate range: ~0.2 – 0.4 mL/min

  • Lower backpressure compared to smaller particle columns

  • Suitable for standard HPLC systems with low‑flow capability

  • Compatible with UV and MS detection

✅ Important: Ensure system can deliver stable low‑flow performance

Why Choose HPS Silica™ Columns

High‑Purity Unmodified Silica

  • Ideal for normal phase chromatography

  • Strong interaction with polar compounds

USP L3 Compatibility

  • Suitable for regulated and compendial methods

  • Reliable alternative to other silica columns

Reproducible Performance

  • Consistent selectivity across batches

  • Proven reliability in long‑term use

Performance Advantages

Lower Backpressure (5 µm Particles)

  • Enables higher flow rates

  • Compatible with standard HPLC systems

Fast Equilibration

  • Reduces time between runs

Excellent for Polar Compounds

  • Strong retention in normal phase mode

  • Ideal for lipids and hydrophilic analytes

Typical Applications

  • Fats and lipid analysis

  • Normal phase HPLC methods

  • Polar compound separations

  • USP L3 method compliance

  • Food, pharmaceutical, and chemical analysis

Important Usage Notes

  • Designed for normal phase chromatography conditions

  • Use appropriate non‑polar mobile phases

  • Requires proper solvent preparation and system equilibration

  • Store and handle using standard silica column protocols

 

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